(87 month(s) ago), sir plz send me this presentation plz plz ,my mail is, csanten7@gmail.com, By: The on-site assessment is a peer review conducted by a member of AAALAC’s Council on Accreditation and at least one ad hoc Consultant/Specialist. The average life span is 8-12 years. dianeribuot Aim:- To study about commonly used instrument in Experimental pharmacology 1.Student Organ Bath:- Tissue bath used to put the animal tissue for studying the drug action. Completely or partially agree = 61%. Based on your opinion, S7A safety pharmacology endpoints in … 8. Find specific details on this topic and related topics from the Merck Vet Manual. Objective: To evaluate pharmacy students' perceptions, barriers, and confidence regarding animal use in the pharmacology laboratory course, and the effect of animal handling on their confidence. I Important experiments were effect of drugs on frog heart, rabbit gut, rabbit eye, etc. In 1996, the name was changed to underscore that AAALAC International accredits institutional animal care and use programs around the world. Sample documents, which institutions can use to facilitate Policy implementation and document institutional compliance, can be downloaded from the OLAW Web site, including the Animal Welfare Assurance of Compliance, OLAW Annual Report, and Semi-annual Program and Facility Review Checklists. Laura A. Conour, ... Lynn C. Anderson, in Anesthesia and Analgesia in Laboratory Animals (Second Edition), 2008. Enter one or more tags separated Copyright © 2021 Elsevier B.V. or its licensors or contributors. Most controlled substances used in laboratory animals are for anesthesia, analgesia, and euthanasia purposes. Study of gastric secretions Conducting experiments on circulationCAT: CATEXPERIMENTAL USES : EXPERIMENTAL USES To study physiology of circulatory and neuro muscular systems Study of drugs affecting BP Investigations of ganglionic blockersMONKEY: MONKEY (Macaca mulatta)EXPERIMENTAL USES : EXPERIMENTAL USES in the field of virology, parasitology, immunology and in immunosuppression, nutrition, reproduction etc.BLOOD COLLECTION & ADMINISTRATION OF FLUIDS & DRUGS: BLOOD COLLECTION & ADMINISTRATION OF FLUIDS & DRUGSDRUG ADMINISTRATION TECHNIQUES: DRUG ADMINISTRATION TECHNIQUES Route - nature of the agent ,animal & the purpose of the administration Oral (p.o) - by addition to the food/water Gavage needles : Through mouth into stomach or lower esophagus Exact amount administered Amount limited to 1% of body weightPowerPoint Presentation: MEASURE FROM TIP OF NOSE TO LAST RIB TO DETERMINE PROPER INSERTION DEPTHPowerPoint Presentation: Mice, rat - doesn’t require anesthesia Rabbit - restrainer used. Dynamic All rights reserved. Additional training materials accessible on the OLAW Web site include audiovisual materials such as “Working Safely with Nonhuman Primates,” “Working with the Laboratory Dog-Training for the Enhancement of Animal Welfare in Research,” and “Training in Survival Rodent Surgery.” The Web site offers a direct link to the Veterans Administration Office of Research and Development's training program entitled, “ResearchTraining. Copy, Automatically changes to Flash or non-Flash embed, WordPress Embed In this scenario, the laboratory animal veterinarian would maintain all controlled substance inventory, administration, and disposal records related to laboratory animal medical care, while individual researchers or departments would maintain their own state controlled substance licenses and DEA registrations for approved research. EQUIPMENT AND INSTRUMENTS Electronic Balances Pharmacology Practical is created specially for the Diploma, Under graduate and Post graduate students of Pharmacy. Systematic Review of Chimpanzee Use in Monoclonal Antibody Research and Drug Development: 1981-2010, Raija Bettauer. The articles, which describe OLAW's past and present interpretation and implementation of Policy provisions, provide a valuable source of information that demonstrates the historical evolution of Policy issues and methods proposed to address implementation and compliance. rsharma524 Necessary measures should be taken to prevent environmental contamination by microorganisms held by laboratory animals. Laboratory animals are currently used by government and private academic institutions, national research institutes, local pharmaceutical companies, and clinical research companies for research, instructional, and testing purposes. These training opportunities also serve as open forums for discussion of regulatory, compliance, and management issues. The AAALAC International is a private, nonprofit organization that promotes the humane treatment of animals in science through a voluntary accreditation program, a program status evaluation service, and educational programs. (100 month(s) ago), hi i am suerprise about your well and good presentation, WELCOME: WELCOMEEXPERIMENTAL ANIMALS IN PHARMACOLOGY: EXPERIMENTAL ANIMALS IN PHARMACOLOGY Neena ThomasCONTENTS: CONTENTS INTRODUCTION EXPERIMENTAL ANIMALS HANDLING AND RESTRAINT DRUG ADMINISTRATION BLOOD COLLECTION CONCLUSION REFERENCESINTRODUCTION: INTRODUCTIONEXPERIMENTAL ANIMALS: EXPERIMENTAL ANIMALS Mice Rat Rabbit Guinea pig Hamster Dog Cat Monkey Frog BIOMETHODOLOGY OF MOUSE (mus musculus): BIOMETHODOLOGY OF MOUSE ( mus musculus) Order-RodentiaPowerPoint Presentation: GENERAL BIOLOGY Smaller docile Acute hearing, well developed sense of smell, poor vision & short generation interval circadian rhythm Swiss albino mouse - widely used strainBASIC BIOLOGIC DATA: BASIC BIOLOGIC DATA ADULT BODY WEIGHT:MALE 20-40 gm ADULTBODY WEIGHT:FEMALE 18-35 gm LIFE SPAN 1.5-3 yrs FOOD CONSUMPTION 15gm/100gm/day WATER CONSUMPTION 15ml/100gm/day BREEDING ONSET:MALE 50days BREEDING ONSET:FEMALE 50-60days GESTATION PERIOD 19-21days BODY TEMPERATURE 36-37 0 CPHYSIOLOGICAL DATA: PHYSIOLOGICAL DATA HEART RATE (beats/min) 330-780 RESPIRATORY RATE per min 84-230 TIDAL VOLUME (ml) 0.1-0.23 Hb(g/100ml) 10-19 RBC(million/mm 3 ) 4.9-12.5 WBC(thousands/mm 3 ) 4-12 BLOOD VOLUME(%B.W) 7-9HANDLING & RESTRAINT: HANDLING & RESTRAINT GENERAL PRINCIPLES: Use of proper restraint & handling techniques Acclimation period of upto 1 week Handle animals gently & firmly Use restraint devices when appropriate Chemical restraint for prolonged or potentially painful procedures MICE: MICE Grasp the middle of the tail between the thumb & index finger,& lift the mousePowerPoint Presentation: HOW TO PICK UP A MOUSE WITH THUMP FORCEPS Forceps tip covered with rubber/polyethylene tubing Grasp the middle of the tail between the forceps Too much pressure avoidedFOR TREATMENTS & CLOSE EXAMINATIONS : FOR TREATMENTS & CLOSE EXAMINATIONS held by tail & placed on a rough surface Grasp the loose skin over neck & shoulder with the thump & fingersRESTRAINT DEVICES: RESTRAINT DEVICES adapted to the restraint devices regularly monitored access to food or water released from restraint devices at least daily easy to clean & provide adequate ventilationMOUSE RESTRAINER: MOUSE RESTRAINER Different types available Plexiglasses, plastic cones,etc.SEXING: SEXING distance from the anus & genital papilla is greater in malesEXPERIMENTAL USES: EXPERIMENTAL USES Acute toxicity studies Assay of insulin and analgesics in genetics and cancer research Testing of drug for teratogenicity BIOMETHODOLOGY OF RAT (rattus norvegicus) order-Rodentia: BIOMETHODOLOGY OF RAT (rattus norvegicus ) order-RodentiaPowerPoint Presentation: GENERAL BIOLOGY Albino rat - widely used 2 strains: wistar sprague dawleyBASIC BIOLOGIC DATA: BASIC BIOLOGIC DATA ADULT BODY WEIGHT:MALE 300-500 gm ADULT BODY WEIGHT:FEMALE 250-300gm LIFE SPAN 2-3.5 yrs FOOD CONSUMPTION 10gm/100gm/day WATER CONSUMPTION 10-12ml/100gm/day BREEDING ONSET:MALE 65-110days BREEDING ONSET:FEMALE 65-110days GESTATION PERIOD 21-23days BODY TEMPERATURE 38-39 0 CPHYSIOLOGICAL DATA: PHYSIOLOGICAL DATA HEART RATE (beats/min) 300-500 RESPIRATORY RATE per min 65-180 TIDAL VOLUME (ml) 1.5 Hb(g/100ml) 12-17.5 RBC(million/mm 3 ) 7.2-9.6 WBC(thousands/mm 3 ) 6-12 BLOOD VOLUME(%B.W) 6-7HANDLING & RESTRAINT: HANDLING & RESTRAINT Grasp the base of tail wit thumb & forefinger FOR HEAVY RATS FOR SMALL RATSFOR CLOSE TREATMENTS/EXAMINATIONS: FOR CLOSE TREATMENTS/EXAMINATIONS Grasp the whole body with palm over the back & restrain forelimbs. AAALAC International has a cooperation agreement with the World Organisation for Animal Health (OIE) to use Chapter 7.8 of the OIE's Terrestrial Animal Health Code (OIE, 2011; http://web.oie.int/eng/normes/mcode/en_chapitre_1.7.8.htm), “Use of Animals in Research and Education” (an AAALAC International reference resource) in its assessments of programs worldwide, with particular emphasis on countries that do not have their own legislative framework for ensuring the welfare of animals used in research, testing, or teaching. Software's used in Experimental Pharmacology (CAL), Application of Biostatistics in Experimental Pharmacology, Experimental animal studies on Analgesic activity, Maitainance, Nomenclature of Genetic Monitoring of Experimental Animal, Endocrine Pharmacology:M.C.QUE'S ON ENDOCRINE PHARMACOLOGY. In its assessments of animal care and use programs, AAALAC relies on Three Primary Standards: the Guide (NRC 2011a); the Guide for the Care and Use of Agricultural Animals in Research and Teaching (Ag Guide: FASS, 2010); and the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Purposes, Council of Europe (ETS 123) 1986. MyersJr., in Research Regulatory Compliance, 2015. Blood Collection From Saphenous VeinPowerPoint Presentation: The mouse is restrained in a plastic tube with air holes in the end.PowerPoint Presentation: Shaving Avoid cutting the skin by holding the blade as flat as possible.PowerPoint Presentation: The vein is now easily visible.PowerPoint Presentation: vein punctured with a 27-gauge needle held at approximately 90 degrees to the skin.PowerPoint Presentation: Bleeding Immediately after puncture a drop of blood will appearPowerPoint Presentation: Blood collection blood collected in a sampling tube or microhaematocrit tube 100 microliters can be collectedPowerPoint Presentation: TOE CLIPPING/ TAIL CLIPPING Unacceptable method CARDIAC PUNCTURE Equipment : 0.9-0.5mm needle Terminal blood collection Requires anesthesia Withdraw blood slowly Upto 1ml recoveredPowerPoint Presentation: ORBITAL SINUS VENIPUNCTURE Equipment : capillary tubes Convenient & easy method Upto 0.5ml can be recovered Tube directed into orbital sinus Inserted into medial canthus of eye Requires anesthesiaPowerPoint Presentation: Use a sharp end glass capillary tube to penetrate the orbital conjunctiva and rupture the orbital sinusPowerPoint Presentation: Collect blood with a vialPowerPoint Presentation: AXILLARY BLEED Equipment : scalpel blade,3-5cc syringe Blood recovered from axillary region in a terminal exsanguination Requires anesthesia 1-2ml of blood recoveredPowerPoint Presentation: RAT Lateral tail vein venipuncture : method same as in mice Ventral tail artery Equipment : 22G needle,3cc syringe Anesthesia required Cardiac puncture : same as in mice Orbital sinus venipuncture : same as in micePowerPoint Presentation: RABBIT MARGINAL EAR VEIN: Equipment : butterfly set Small vol of blood collected Sedation & vasodilation with acepromazinePowerPoint Presentation: CENTRAL EAR ARTERY Equipment : plain 20G, 1 inch needle attached to silicone coated tube Volume : 10ml/kg body wt Sedation & vasodilatation with acepromazinePowerPoint Presentation: CARDIAC PUNCTURE Equipment : 18G, 1.5 inch needle attached to vacuum tube Terminal blood collection Requires anesthesiaPowerPoint Presentation: GUINEA PIG LATERAL SAPHENOUS VEIN VENIPUNCTURE Equipment : 22G needle/ scalpel blade Small vol blood collected LATERAL SAPHENOUS VEIN(VACUUM ASSISTED) Larger vol blood collected META TARSAL VEIN TOE CLIPPING Unacceptable methodPowerPoint Presentation: CRANIAL VENACAVA Equipment : 22-23G needle,3-6 cc syringe Site : manubrium of sternum Repetitive collection of >1ml blood (upto 7ml/kg) Anesthesia required CARDIAC PUNCTURE Equipment : 20-22G needle,12-20cc syringe Not recommended as a repetitive blood sampling procedure Requires anesthesiaCONCLUSION: CONCLUSIONREFERENCES : REFERENCES Fundamentals of Experimental Pharmacology . The common laboratory animals are rat, mice, guinea pig, rabbit frog & hamster. The specific issues in these program components were as follows: Kathryn Bayne MS, PhD, DVM, Lynn C. Anderson DVM, DACLAM, in Laboratory Animal Medicine (Third Edition), 2015. OLAW has issued a notification that PHS-Assured institutions outside the U.S. are required to implement the revised Guiding Principles (http://grants.nih.gov/grants/guide/notice-files/NOT-OD-13-096.html). How can I obtain a copy for a class I will be teaching next week? Training workshops and symposia are offered regionally and are often cosponsored with organizations such as the Scientists Center for Animal Welfare (SCAW), the National Science Foundation (NSF), the Public Responsibility in Medicine and Research (PRIM&R), ARENA, the Animal Welfare Information Center (AWIC), the Institute for Laboratory Animal Research (ILAR), the American Association for Laboratory Animal Science (AALAS) and the American College of Laboratory Animal Medicine (ACLAM), in conjunction with state biomedical research association organizations and local academic institutions. Submissions from 2005 PDF Animal testing has been a contentious issue in biomedical research for a long time. Laboratory animal veterinarians and animal care program directors historically have supplied and managed controlled substance activities for nonclinical research due to their inherent responsibilities for managing animal care and health and regulatory compliance. Experimental Pharmacology, Volume 2 . Integrated summaries of safety and efficacy. In experimental pharmacology and toxicology the aim is to observe for effects induced by chemicals in animals. Realizing the enormous need for improving the standards and logistics of meeting the national requirements of laboratory animals in the United States, the Division of Biology and Agriculture of the National Research Council—National Academy of Sciences in 1952 established the Institute of Laboratory Animal Resources (ILAR). If all aspects of experimental design are not thoroughly addressed, scientists may reach false conclusions and pursue avenues of research that waste considerable time and resources. Recently, the Philippine College of Laboratory Animal Medicine (PCLAM), an affiliate of the Philippine Veterinary Medical Association (PVMA), was established in 2015 to further strengthen responsible animal use and care with its mission of training more veterinarians as specialist in the field of laboratory animal medicine and science to further laboratory animal research in the country. Dogs used for research or teaching may be purpose-bred. The veterinarian should also contribute to the establishment of appropriate policies and procedures for ancillary aspects of veterinary care. As a result, laboratory animal veterinarians should avoid dispensing or distribution of controlled substances unless their state regulatory boards and the DEA approve such practices. For example, in the Pacific Rim region, the three most commonly identified mandatory deficiencies are related to (in rank order): (1) IACUC; (2) animal environment, housing, and management; (3) program of veterinary care. In addition, some states require that all dispensed controlled substances be reported to a central state managed database for diversion prevention and control. The AAALAC International is a private, nonprofit organization that promotes the humane treatment of animals in science through a voluntary accreditation program, a program status evaluation service, and educational programs. Laboratory animal research in the Philippines started as early as the 18th century when Spanish conquistadors sent a mission to the country to study smallpox. AAALAC International is in a unique position to observe and benchmark the standards of laboratory animal care and use around the world given its presence in 34 countries. In order to view it, please For decades, scientists have used animals to carry out tests before they implement them on humans. Predictive in vivo animal models and translation to clinical trials, Natalie Cook, Duncan I. Jodrell, and David A. Tuveson. Drug discovery and evaluation . 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